Methods: The microstructure of cystic concretion was observe using B-ultrasonography and the serum albumin concentration was determined using biuret reagent colorimetric method and bromcresol green colorimetric method. 方法:用B型超声检查,电子扫描观察胆囊结石的细微结构,采用双缩脲法和溴甲酚绿方法定量测定血浆白蛋白的含量,对298例肝硬化合并胆囊结石病人资料进行临床分析。
Objective For conquering the interference of lipid turbidity, jaundice and hemolysis, changed simple reagent of biuret to double reagent ( the method). 目的为克服乳浊、黄疸、溶血对血清总蛋白测定的影响,将双缩脲法改为双剂型方法。
Investigation and application of measuring serum total protein by improving biuret double reagent through two point terminal method 改良双缩脲双试剂二点终点法测定血清总蛋白的研究与应用
Methods The first reagent was blank biuret reagent without cupric sulfate and cupric sulfate of second reagent was double than original reagent. 方法第一试剂为不含硫酸铜的双缩脲空白试剂,第二试剂系将原双缩脲试剂中的硫酸铜增加一倍,二者等量混合即成原法试剂。